When to Begin Testing
You may test at any time of the day, but you should test at
approximately the same time each day. Reduce your liquid intake
for 2 hours before testing. To find out when to begin testing,
determine the length of your normal cycle. The length of your
cycle is from the beginning of one period (the day of first bleeding)
to the beginning of the next. Count the first day of bleeding
or spotting as day one (1). If your cycle length is irregular,
that is, if it varies by more than a few days each month take
the average number of days for the last 3 months. Use the chart
shown to work out the day you should begin testing. The day you
begin testing is listed opposite the number of days in your normal
of Normal Cycle
|Start testing this many days after your
last period began.
of Normal Cycle
|Start testing this many days after your
last period began.
For example, if your period normally begins every 28 days,
you should begin testing eleven (11) days after the beginning
of your last period.
Note in the calendar above: If the ninth ( 9th) in the
calendar above is the first day (day one *) of menstrual
bleeding, then the 19th, or day eleven, of your cycle is the
day to begin testing (s).
- Note if the expiration
date is valid and remove the LH One-Step Ovulation Test Strip
from the foil pouch.
- Holding the strip vertically
with the absorbent pad end down, carefully dip the test into
the urine specimen for approximately 6- 8 seconds or until you
begin to see the migration of the sample into the result area.
Do not immerse the strip past the stop line.
- Lay the LH One-Step
Ovulation Test Strip on a flat surface with the result area facing
up and wait 3-5 minutes for the results to appear. After the
5 minute mark, the test becomes invalid. Do not read the results
after this point.
- After interpreting
the test results, discard the test strip.
Interpretation of the Test
Interpret the test result at approximately 3 to 5 minutes.
As the LH One Step Ovulation Test Strip begins to work,
a colored band will appear in the upper section of the result
area to show that the test is working properly. This band is
the Control Band (C). The lower section of the result area indicates
the test results. If another colored band appears at the lower
section of the result area, this band is the Test Band (T) and
indicates a positive test.
The presence of two similar, purple-colored bands (equal
darkness) within the result area, no matter which one appears
first, means that an increase in LH hormone is detected (See
results matrix). The color of the Test band should be the
same as, or darker than, the Control Band to indicate a positive
test for LH surge.
PLEASE NOTE!!! The presence of a purple Control Band and a lighter
colored, or fainter, purple Test Band means that the
LH hormone increase is not detected. The presence of only
one purple color band (Control Band) within the result area
also indicates that the LH surge has not occurred.
If, after performing the test, no purple color band is
visible anywhere within the result area, the result is considered
invalid. The directions may not have been followed correctly
or the test may have deteriorated. You have to repeat the test
by using a new LH One-Step Ovulation Test Strip.
Function of the Control Band:
The Control Band is used as a reference and built in quality
control check. If the Test Band is darker or similar to the Control
band, the test result is considered positive. If the Test Band
is lighter than the Control Band, the test result is considered
negative. The Control Band is also used for procedural control
to check whether the test reagents are working properly and that
a sufficient amount of urine sample has been applied to the absorbent
How to Recognize the LH Surge:
After reading the results of each test, you must decide it you
are having your LH surge. If your test result is positive, you
are probably experiencing your LH surge. A LH surge can last
from one to three days depending on your cycle and other biological
factors. If you have technical questions or you cannot determine
a LH surge after several months, you should seek professional
advice. If your test result is negative, you are probably not
having your LH surge. Remember that a pink/rose test band lighter
than the control band shows that there is only a very low level
of LH in your urine.
When to Stop Testing: Unless otherwise specified by
your doctor, stop testing once the LH surge is detected.
Limitations of the Test
Q: How accurate is the LH One-Step Ovulation Test Strip?
A: The LH One-Step Ovulation Test Strip has been shown
to be over 99/o accurate in laboratory testing and 99% accurate
in consumer testing.
Q: When can I do the test?
A: After you determine the best day to start testing
based on your monthly cycle, you can do the test any time on
that day. Testing with first morning urine is not recommended.
Testing between 10 am and 8pm is a good time frame, with many
REs suggesting testing at 10am. Test at about the same time each
day. Reduce your liquid intake for 2 hours before testing.
Q: What if no band appears in the result area?
A: If no band appears in the result area after 5 minutes,
the result is invalid and you should repeat the test with another
LH Ons-Step Ovulation Test Strip.
Q: I have tested for five days and I did not see a surge.
What do I do?
A: Since every woman does not always ovulate at mid-cycle,
you may not detect the LH surge in the first five days of testing.
This could mean you have not ovulated yet and you should continue
testing with additional LH One-Step Ovulation Test Strips.
Q: I have tested for 8 days or more and I still did not
see my LH surge, what is wrong?
A: About 90% of ovulating women with regular cycles
will see their surge during 8-10 days of testing. If you do not,
it could mean that you have not ovulated this month. lf you are
having a short cycle this month, it is possible that you have
ovulated before you started testing. If you are having a long
cycle this month, you may not have ovulated yet. You may continue
testing or test again next month. Don't worry, it is normal to
have an occasional short or long cycle, or to have a cycle without
Q: I have used the ovulation test for three months, and
each month I have seen a surge and have had intercourse that
day or night. I have not become pregnant yet. What is wrong?
A: First, it is important to remember that it can take
normal, healthy couples many months to become pregnant. There
are many factors that can affect your ability to become pregnant
even if you have been able to have intercourse during your most
fertile days. If after several months you have no success, consult
with your physician or healthcare provider.
Q: What could interfere with my test result?
A: If you are actually pregnant, have recently been
pregnant, or have reached menopause you may get a misleading
result. Some prescription drugs, such as menotropins for injection
and danazol may affect the results you see. Please consult your
physician if you have any questions about prescription drug interactions.
Q: Will oral contraceptives affect the result?
A: After using the pill your cycle may be irregular
and may take some time to stabilize again. You may wish to wait
until you have had two normal periods before starting to use
the LH One-Step Ovulation Test Strip.
BACK to Test page
OVULATION (LH) TESTS
One Step LH Urine Ovulation Test is fast and easy-to-use. It
is a qualitative test that predicts LH (Luteinizing Hormone)
surge, and in turn, when you are likely to ovulate and conceive.
Females who are trying to become pregnant may use this information
to improve their chances of
conception. Luteinizing Hormone is a hormone released by the
pituitary gland, a small gland at the base of the brain. The
hormone stimulates the ovaries to produce and release eggs each
month during the menstrual cycle. The level of LH in the blood
is highest before ovulation. This increase in hormone level is
sometimes called a "surge". If fertility drugs are
given to stimulate ovulation, an LH test can help determine the
best time for sexual intercourse. The LH test may also be used
to determine when eggs are mature enough to be surgically removed
from the ovary as part of the in vitro fertilization process.
LH tests may also aid in the diagnoses of polycystic
ovary disease, premature ovarian failure, and menopause.
The tests detect LH in urine in a qualitative format with
sensitivity of 20mIU/mL (International Standard Value) and specificity
is 99.8%. Tests have an expiry date of 2 years and can be stored
in a dry environment ranging from 2 to 30 degrees Celsius. Results
are available in just minutes.
WHEN TO BEGIN TESTING
First, you must determine the length of your menstrual cycle.
This is the number of days from the first day of your menstrual
bleeding to the day before your next bleeding begins again. Please
refer to the chart below to determine when you should start testing.
If your cycle is shorter than 21 days or longer than 38 days,
consult your doctor. If you do not know your cycle length, you
may begin the test 11 days after your first period since the
average cycle length is 28 days. Perform 1 test each day over
a 5 day period or until the LH surge has been detected.
1. Do not use first morning urine samples as LH is synthesized
in your body early in the morning. It will not show up in your
urine until later in the day.
2. The best time to collect your urine is between 10am - 8pm.
3. Collect urine at about the same time each day.
4. Reduce liquid intake about 2 hours before collecting your
urine as a diluted urine sample can prevent the test from detecting
Please refer to the table below for information on when to
start using ovulation tests:
Your Cycle Length Start
To Test On
21 days Day
23 days Day
24 days Day
26 days Day
27 days Day
28 days Day
29 days Day
30 days Day
37 days Day
38 days Day
Bring the test pouch to room temperature (18-30°C). Collect
urine into a clean container (such as plastic cup). The best
way to collect sample is by placing a cup in the middle of urination
process. To begin testing, open the sealed pouch by tearing along
the notch. Remove the test from the pouch when you are ready
to use it.
Immerse the strip into the urine with the arrow end pointing
towards the urine. Do not immerse past the MAX (maximum) line.
Take the strip out after 5 seconds and lay the strip flat on
a clean, dry, non-absorbent surface (e.g., mouth of the urine
container). Do not immerse for longer than 7 seconds.
Wait for colored bands to appear. Depending on the concentration
of LH in the test specimen, positive results may be observed
in as short as 40 seconds. However, to confirm negative results,
the complete reaction time (10 minutes) is required. Do not read
the results after 30 minutes as this type of test is designed
for rapid determination only.
INTERPRETATION OF RESULTS
No LH Surge: Only one color band appears
on the control region or the test
band is present but lighter in color intensity than the control
band. There is no LH surge even if two
lines are present - as long as the test
line is fainter than the control line- the result is negative.
LH Surge: If two color bands are
visible and the test band
is equal to or darker than the control band, one will
probably ovulate in the next 24-48 hours. If one wants to be
pregnant, the best time to have intercourse is after 24 but before
Invalid - Uncertain Results: No visible bands in the control
and test regions. Make sure to follow the above specified instructions
for optimum results. The intensity of the test line is directly
proportional to the concentration of LH in urine.
Test results may vary for different individuals depending
on the concentration of LH your body produces, as well as monitoring
frequency, and testing technique. The best method is to test
once a day, preferably later in the afternoon or early evening,
by closely following test instructions.
PRECAUTIONS1. For single in vitro diagnostic use only - use
test only once for urine screening.
2. Do not use test kit beyond expiry date.
3. Keep away from moisture, direct sunlight, and children.
Note: If you suspect that you have ovulation problems, or
problems with detecting LH surge, please see your physician.
There are numerous stimulatory procedures that can induce the